Surgery
of cultivated limbal epithelium which can then be used to treat both eyes. Not only is surgical technique of both limbal biopsy and transplantation simple and sutureless, our technique of limbal cultivation is also rapid and completely avoids the use of animal derived products in cell culture. Over the last decade, significant advances in the reliability of cultivation
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case report of unique application, Cornea, 2003;22(5):478–81.
18. Sangwan VS, Matalia HP, Vemuganti GK, et al., Early results of penetrating keratoplasty after cultivated limbal epithelium transplantation, Arch Ophthalmol, 2005;123(3):334–40.
19. Vemuganti GK, Kashyap S, Sangwan VS, Singh S, Ex-vivo potential of cadaveric and fresh limbal tissues to regenerate cultured epithelium, Indian J Ophthalmol, 2004;52(2):113–20.
20. Fatima A, Sangwan VS, Iftekhar G, et al., Technique of cultivating limbal derived corneal epithelium on human amniotic membrane for clinical transplantation, J Postgrad Med, 2006;52(4):257–61.
21. Zhang X, Sun H, Tang X, et al., Comparison of cell-suspension and explant culture of rabbit limbal epithelial cells, Exp Eye Res, 2005;80(2):227–33.
22. Fatima A, Vemuganti GK, Iftekhar G, et al., In vivo survival and stratification of cultured limbal epithelium, Clin Experiment Ophthalmol, 2007;35(1):96–8.
23. Nakamura T, Inatomi T, Sotozono C, et al., Successful primary culture and autologous transplantation of corneal limbal epithelial cells from minimal biopsy for unilateral severe ocular surface disease, Acta Ophthalmol Scand, 2004;82(4):468–71.
24. Sangwan VS, Matalia HP, Vemuganti GK, et al., Clinical outcome of autologous cultivated limbal epithelium transplantation, Indian J Ophthalmol, 2006;54:29–34.
25. Koizumi N, Inatomi T, Suzuki T, et al., Cultivated corneal epithelial stem cell transplantation in ocular surface disorders, Ophthalmology, 2001;108(9):1569–74.
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